目的 研究不同盐碱胁迫对金丝楸幼苗生长、光合和生理指标的影响，并结合转录组测序分析，探究楸树耐盐碱的生理机制和分子机制。 方法 采用盆栽法对金丝楸幼苗进行不同盐碱胁迫处理，分析其生物量、光合及生理指标对不同盐碱响应的差异，采用Illumina高通量测序技术进行转录组测序，通过生物信息学分析盐碱胁迫对转录水平的影响。 结果 不同盐碱胁迫下，金丝楸幼苗叶片受伤害程度为Na₂CO₃>混合盐碱>NaCl；新增株高和地径、地上部和根的干质量和鲜质量、生物量、根冠比均受到明显抑制，并随盐碱浓度增加而抑制加强，但生长胁迫指数均随浓度的增加而降低；丙二醛（MDA）含量和相对电导率都随胁迫浓度增加而不同程度上升，超氧化物歧化酶（SOD）活性、可溶性糖含量、脯氨酸（Pro）含量、叶绿素总量和光合速率都呈现先上升后下降的趋势。转录组测序共产生约60.4 Gb原始数据，组装得到55 793个Unigenes，其中29 534（52.93%）个Unigenes获得了注释；通过差异表达基因（DEGs）分析，3个比较组（CK vs NaCl，CK vs Na₂CO₃ 和 CK vs 混合盐碱）分别筛选出1 779、2 835和4 059 个DEGs；DEGs GO富集分析表明，膜的整体成分、膜的内在成分、催化活性、类异戊二烯代谢和合成过程、氧化还原酶活性等条目被显著富集；DEGs KEGG分析表明，苯丙素生物合成、淀粉和蔗糖代谢、植物激素信号转导、萜类主干生物合成和精氨酸代谢等通路被显著富集；此外，在DEGs中鉴定的bHLH、ERF、MYB-related、NAC、C2H2、WRKY、MYB和bZIP转录因子家族成员最多。 结论 金丝楸主要通过积累可溶性糖和Pro，提高SOD酶活和光合作用来抵御盐碱胁迫，但都呈现“低促高抑”的现象，说明其具有一定阈值。金丝楸通过调节膜成分、催化活性、类异戊二烯代谢和生物合成过程、苯丙素生物合成、淀粉和蔗糖代谢、植物激素信号转导等生物过程和代谢途径，并结合有关转录因子共同响应盐碱胁迫。本研究为深入研究楸树耐盐碱生理机制和分子机制提供科学的理论依据。

In this study, the rhizosphere soil properties of Takyr Solonetzs ameliorated with FGDG, and its effect on root growth of Lycium barbarum were studied. The results indicated that with increasing applications of FGDG, the contents of Na+, K+, Mg2+, Cl-, and HCO3- in rhizosphere soil decreased gradually with the ratio of 51.9%, 16.7%, 42.7%, 44.7% and 28.2%, respectively, while the contents of Ca2+ and SO42- showed an increasing trend and increased by 96.9% and 80.4% compared with those of the CK. As the content of exchangeable Na+ decreased with the increasing application of FGDG, the exchangeable sodium percentage (ESP) and soil pH values decreased from 32.2% and 10.5 to 18.1% and 7.82, respectively and thus the soil physical properties improved. However, the lowest total salt content (2.91 g·kg-1) was found in T2 (2.5 t/(667m2)), and similarly, the highest values of taproot length (44.78 cm), root surface area(179.97 cm2) and root dry weight (17.53 g) were also found in T2, which suggested that applications of FGDG were not always positively correlated with soil reclamation, and excessive amount of FGDG would inhibit root’s growth. Overall, application of FGDG is effective in ameliorating Takyr Solonetzs, but high amount of FGDG would result in excess salts against root growth and subsequently influence production.

The regenerated p lantlets of Acacia auriculiform is were obtained by the method of re2differentiation of thecallus from the hypocotyls exp lants. The effects of p lant regulator compositions on the induction of callus and thedifferentiation of adventitious buds in vitro culture were studied. The op timum medium for callus induction wasMSmedium containing 1. 0 or 1. 5 mg ·L-1 2, 42D and 0. 5 mg ·L-1 KT and 100% the callus induction frequencywasobtained. The op timum medium for re2differentiation of calluswasMSmedium containing 1. 5 mg ·L-1 62BA and0. 2 mg ·L-1 NAA and 84. 7% of adventitious shoot regeneration frequency with 5. 83 shoots per exp lants wasobtained. In addition, the op timum medium for the adventitious buds induced from the hypocotyls exp lants withouttransferring the calluswasMSmedium containing 2. 0 mg ·L-1 62BA, 0. 1 mg ·L-1 NAA and 0. 2 mg ·L-1 KT.Excised shootswere effectively elongated inMS medium without appending any hormones. Elongated shoots of 3. 0cm rooted when theywere transferred toMSmedium containing 0. 1 mg ·L-1 IAA and 0. 2 mg ·L-1 NAA after 30days and developed into healthy p lantlets, which resulted in a rooting rate of 85 %. The results of this study willfacilitate the app lication of genetic transformation methods in A. auricu liform is.

以日本落叶松杂交Solexa转录组测序获得的数据为依据,经拼接后获得尿苷二磷酸葡萄糖脱氢酶(UDPGDH)原序列,以该序列两端设计引物,在落叶松亲代和子代幼嫩针叶中成功克隆了UDPGDH基因cDNA序列,序列长1 443 bp,编码480个氨基酸。qRT-PCR结果分析表明:UDPGDH基因的表达量在落叶松杂交子代中远远高于亲本,正交子代的表达比反交子代高,说明UDPGDH在形成细胞壁半纤维素前体物过程中起着非常重要的作用,暗示UDPGDH在子代的高表达有利于杂种优势的形成。

目的 量化黄河故道刺槐人工林结构多样性，为黄河故道森林结构精细调控和森林质量精准提升提供依据。 方法 对2块50 m × 100 m黄河故道刺槐人工林固定样地进行每木定位和调查，采用径阶-林分空间结构参数二元分布同时量化描述林木空间和大小结构特征，并采用α多样性指数量化描述不同空间结构状态下林木大小多样性。 结果 刺槐人工林林分A和B中6～36 cm径阶随机分布林木分别占56.2%和52.2%，且随机分布林木具有更高的大小多样性（H_A = 2.73、H_B = 2.75）和较差的均匀性。不同优劣等级林木径阶分布基本呈正态分布，且林木越粗壮越占竞争优势，但林分B中不同优劣等级林木具有更高的大小多样性。林分A和B中分别有85.1%和80.5%的林木混交较差，较集中分布于8～30 cm和14～34 cm，林分B中不同混交度等级林木具有更高的大小多样性。林分A中非常稀疏林木径阶分布呈右偏正态分布，其6～30 cm占到37.2%，而林分B非常密集林木径阶分布呈双峰型，其6～8 cm和22～40 cm的株数比例分别为8.7%和22.5%。 结论 黄河故道刺槐人工林不同空间结构和大小多样性较差，需制定有效经营措施以提高森林生产力。